Coding

Part:BBa_K2272016:Design

Designed by: Eric Joshua Wang   Group: iGEM17_Virginia   (2017-10-22)


pMnSOD-Cytochrome(Cyt)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1041
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 244
    Illegal NgoMIV site found at 259
    Illegal NgoMIV site found at 993
    Illegal NgoMIV site found at 1065
    Illegal NgoMIV site found at 1113
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1640
    Illegal BsaI.rc site found at 1796


Design Notes

The MnSOD promoter and the cytochrome coding sequence was ligated with SCAR.


Source

https://parts.igem.org/Part:BBa_K2272001 https://parts.igem.org/Part:BBa_K2272003

References